The Fluorescent Particle Spray Droplet Tracer Method1
Author: HIMEL, CHESTER M.
Source: Journal of Economic Entomology, Volume 62, Number 4, August 1969 , pp. 912-916(5)
Publisher: Entomological Society of America
Abstract:The fluorescent particle (FP) spray droplet tracer method makes it possible to identify pesticide spray droplets by size and by number directly on insects, foliage, and other solid substrates. All of the insects and the foliage act as impingement substrates for the spray droplets traced by use of fluorescent particles. Spray droplets larger than the range of 10-20-µ, diameter can be detected for the first time in the field on target (and nontarget) insects and foliage. The FP method makes data available on the transport, distribution, and impingement of pesticide sprays. Such data have not been readily available in the past.
The FP method is based on the uniform suspension of a known number of solid, insoluble, micron-size, fluorescent (Zn-Cd sulfide) particles in a known volume of nonvolatile pesticide liquid. The spray droplets formed from such a fluid suspension contain a predictable number of fluorescent particles as a function of the diameter of the droplets at their moment of formation. When a spray droplet containing these insoluble fluorescent particles impinges on target (or nontarget) insects or foliage, the FP's, being insoluble, are filtered out on the surface where they remain with excellent adhesion in a discrete, recognizable droplet grouping. The FP's in each droplet grouping can be counted under a microscope when the substrate is illuminated with long-wavelength UV light. The lower limit of detect ability of spray droplets by size is a function of the number of FP's per unit volume which can be suspended in the original spray liquid while retaining its viscosity and flow characteristics within operational limits.
FP methodology can be independent of use of insecticides and therefore is a new method for large or small area tests on arthropod migration.
Document Type: Research Article
Publication date: 1969-08-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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