Initial Studies on the Extraction of the Active Substance Inducing Monocoitic Behavior in House Flies1, Black Blow Flies2, and Screw-Worm Flies2,3

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A monocoitic substance extracted from male house flies, Musca domestica L., the black blow fly, Phormia regina (Meigen); and the screw-worm fly, Cochliomyia hominivorax (Coquerel), prevented mating when it was injected into virgin female house flies. The active substance present in the male house fly and screw-worm fly can be extracted with water, methanol, and 95% ethanol. It is more soluble in methanol than in abso- lute ethanol, and it is insoluble in diethyl ether and chloroform. Dialysis and chromatography on Sephadex® indicated mat it has a low molecular weight. The activity of extracts from the male house fly was not reduced by heating in a boiling water bath for 2 min. Thus, the substance is polar, of a relatively small molecular weight, and not a lipid or protein.

Document Type: Research Article

Publication date: June 1, 1969

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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