A Method of Analyzing the Milk and Feces of Cows for Coumaphos and Its Oxygen Analog After Feeding Coumaphos for Control of House Fly Larvae1,2

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Abstract:

Milk and feces from dairy cows fed coumaphos (0-44 ppm in air-dried feed) to control house fly larvae, Musca domestica L., were analyzed for coumaphos and its oxygen analog. Coumaphos was recovered only from feces of animals fed this compound while the oxygen analog was undetected. The extracts of feces and milk were analyzed by separating the 2 compounds by liquid chromatography on silica gel. hexane-acetonitrile partition, and gas chromatography of an aliquot of each fraction on a column containing 10% DC-200 on Gas Chrom® Q. The compounds were detected with a phosphorus-sensitive flame photometric cell. Sensitivity of the analysis was better than 0.003 ppm for milk and 0.005 ppm for feces.

Larval control was incomplete but was related to the level of coumaphos fed. Potasan® (O,O-dicthyl O- (4- methyl-2-oxo-2H-1-benzopyran-7-yl) phosphorothioate), which was found in small amounts in the feces, is very likely a metabolite of coumaphos. Its contribution to the larvicidal activity of the feces is not considered significant.

Document Type: Research Article

Publication date: April 1, 1968

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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