Use of Daphnia magna for the Microbioassay of Pesticides. II. Comparison of Microbioassay with Gas Chromatography for Analysis of Pesticide Residues in Plant Extracts1

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Specimens of Daphnia magna Straus, which were killed by extremely small quantities of pesticides, were killed also by small amounts of extracts of common plants, such as lettuce, radishes, and beets. Even following rigorous cleanup, these plant extracts were toxic enough to the test organism to mask any mortality caused by pesticide residues.

Using purified extracts of carrot tops, which had low toxicity to daphnids, the microbioassay procedure for measuring DDT, ethion, and lindane residues was compared with gas-liquid chromatography (GLC). GLC was found to give much more consistent and accurate results than microbioassay with D. magna.

Document Type: Research Article

Publication date: October 1, 1967

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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