Use of Daphnia magna for the Microbioassay of Pesticides. I. Development of Standardized Techniques for Rearing Daphnia and Preparation of Dosage-Mortality Curves for Pesticides1
Authors: FREAR, DONALD E. H.; BOYD, JOHN E.
Source: Journal of Economic Entomology, Volume 60, Number 5, October 1967 , pp. 1228-1236(9)
Publisher: Entomological Society of America
Abstract:Detailed study of optimal conditions of rearing and testing Daphnia magna Straus showed that it was possible to rear this organism under closely controlled laboratory conditions. Under these conditions asexual reproduction yielded a homogeneous population well suited for microbioassay of pesticide residues.
It was found that some pesticides were extremely toxic to daphnids at concentrations as low as a few parts per trillion in water. Most of the pesticides tested showed LD50 values below parts per billion. Directions for rearing daphnids and using them for bioassay are given, with dosage-mortality curves and LD50.values for 56 of the more common pesticides.
Document Type: Research Article
Publication date: October 1967
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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