Rearing Stored-Product Insects for Laboratory Studies: Lesser Grain Borer, Granary Weevil, Rice Weevil, Sitophilus zeamais, and Angoumois Grain Moth1,2,3,4

Authors: STRONG, R. G.; SBUR, D. E.; PARTIDA, G. J.

Source: Journal of Economic Entomology, Volume 60, Number 4, August 1967 , pp. 1078-1082(5)

Publisher: Entomological Society of America

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Cultures of Rhyzopertha dominica (F.), lesser grain borer; Silophilus granaries (L.), granary weevil; S. oryzne (L.), rice weevil; S. zeamais Motschulsky; and Sitotroga cerealella (Olivier), Angoumois grain moth, were reared in a room maintained at 80±2°F and 55±5% relative humidity on Ramona wheat contained in gallon jars. The moisture of wheat used as media was adjusted to contain 13±0.3% moisture content. Systematic rotation of the lesser grain borer was based on food consumption; rotation of cultures of the other species was based on the age of insects in each.

A 30-ml measure of lesser grain borer adults (approximately 9000 live insects) emptied onto 2 quarts of wheat resulted in an average of 29,000 insects per jar in 8 weeks Parent stock of Silophilus spp. was left on 2/½ quarts of media I week, and the same stock was used for 3 consecutive weeks to establish new cultures. Starting with a 40-ml measure of granary weevil adults (approximately 7000) , cultures of this species yielded an average of 10,000 insects in 7 weeks; cultures started with a 27-ml measure of the rice weevil (approximately 5200) and a 30ml measure of S. zeamais (approximately 5200) yielded an average of 12,000 insects in 6 weeks. Adult progeny of the Angoumois grain moth began emerging during the third week after 300 moths were placed on 2½pints of wheat. Emergence reached its peak 5 weeks after initial infestation, and an average of 2850 moths was produced in each culture.

Document Type: Research Article

Publication date: August 1, 1967

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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