Aseptic Rearing of the Codling Moth on Synthetic Diets: Ascorbic Acid and Fatty Acid Requirements1
Author: ROCK, GEORGE C.
Source: Journal of Economic Entomology, Volume 60, Number 4, August 1967 , pp. 1002-1005(4)
Publisher: Entomological Society of America
Abstract:The codling moth, Carpocapsa pomonella (L.), was reared aseptically from newly hatched larvae to adults for 3 successive generations on a synthetic diet containing casein, 2 amino acids, salts, sucrose, vitamins, safflower oil, cholesterol, agar, and water. Growth and development on this diet were comparable to that attained on immature apples.
Elimination of ascorbic acid from the diet resulted in 86% lanai death, mostly first and second instar, and no development to the adult stage. The minimum dietary requirement for ascorbic acid under the reported rearing conditions was between 0.4 and 0.8 g per 100g diet.
Linoleic acid was shown to be an indispensable dietary nutrient; linoleic acid had the same effect as linoleic acid. Diets deficient in both fatty acids resulted in failure of adults to emerge from their pupal cases. Palmitic, stearic, oleic, and methyl arachidonic acids showed no essential fatty acid activity.
Five successive generations were reared in the laboratory on a semisynthetic diet with 55-65% of the larvae developing to normal adult moths. Larval development adult fecundity, and egg viability appeared comparable to that attained for insects reared on immature apples.
Document Type: Research Article
Publication date: August 1967
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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