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Analysis of Male Gypsy Moths for Microgram Quantities of Tepa1

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A method has been developed whereby residues of the chemosterilant tepa can be determined easily to 0.5 g/ gypsy moth, Porthetria dispar (L.). The method involves cleanup of moth extracts by means of column chro- matography followed by colorimetric analysis. Preliminary cleanup attempts with thin-layer chromatography were unsuccessful.

Data obtained indicate that the amount of tepa residues in moths depends on the holding time after treatment. The residues diminish with increasing holding times between treatment and analysis. Washing treated moths with water also has been found helpful in lowering the tepa residue.

In general, it can be stated that the lowest practical level obtainable is in the order of 1 to 5 g. These levels were obtained by washing immediately after treatment followed by a holding period of 0—16 hours.

All residue data were determined on moths which had been exposed for a period of 8 hours to residual films of 1 mg/dm2 of tepa. This treatment is currently considered the most practical for producing sterile moths for field release.

Document Type: Research Article

Publication date: February 1, 1967

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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