Improved Laboratory Methods for Rearing the Boll Weevil1,2

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A disposable bacteriological capillary tube provided a simple and gentle method of implanting egg's of the boll weevil, Anthonomus grandis Boheman, onto artificial diet. The technique is equally adaptable to maintenance of small cultures and to mass-rearing programs.

Eggs were collected from cotton squares (flower buds) by chopping the squares in water with a household food chopper. The eggs were removed, surface sterilized, and submerged in sterile water in a sterile petri dish.

The egg-water suspension was drawn into a disposable bacteriological capillary tube and ejected onto the scratched surface of larval diet in petri dishes. The Petri dishes containing the eggs and diet were held 14-17 days at 26 for development, after which the adults were removed and held in cartons for feeding and egg production.

The moisture content of diet before eggs were implanted was the most important of the conditions investigated in determining the percentage of adults that were obtained.

Document Type: Research Article

Publication date: April 1, 1966

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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