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Surface Sterilization of Eggs of the Boll WeevW with Cupric Sulfate2

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In our 1st attempt to use frozen squares (cotton flower buds) for feeding and oviposition of the boll weevil, Antthonomus grandis Boheman, during the winter, we had extreme difficulty with the growth of microorganisms when eggs treated with 0.01% mercuric chloride were placed on the artificial larval medium. Although the artificial diet of Earle et al. (1959) contains 0.1% sorbic acid and 0.1% methyl-para-hydroxybenzoate, and even though we tried several compounds for surface sterilization of the eggs, we were unable to control microbiological contamination. As a result we obtained very few weevils from contaminated petri dishes.

Document Type: Research Article

Publication date: February 1, 1966

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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