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Integration of Physico-Chemical and Biological Techniques in Specific Bioassay, with Special Reference to Bidrin Insecticide

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Specific bioassay methods for certain insecticides can be developed by the integration of physico-chemical and biological techniques. Based on high insect toxicity, short exposure time, synergistic effect of sesamex, solvent-water partition, and alkali stability, a specific bioassay method was developed for BidrinĀ® insecticide (3-hydroxy-N,N-dimethyl-cis-croton-amide dimethyl phosphate). The overall procedure was formulated by the calculation of the effects of each factor on the recovery of Bidrin and the interference of other insecticides. The mean recovery for Bidrin was 96.0%. Ppm of Bildrin can be detected as low as 0.05 in extracts after cleanup.

The interference of any of 26 organophosphorus, 3 carbamate, and 17 chlorinated insecticides tested is less than 6%; most of them less than 1%. Insecticides which are very soluble in water, such as dimethoate (5.9% interference), trichlorfon (3.5%), phosphamidon (2.5%), mevinphos (1.2%), and IsolanĀ® (1-isopropyl-3-methyl-5-pyrazolyl dimethylcarbamate) (1.6%), gave relatively higher interferences. In most cases, determined and calculated values are in agreement.

From this study several new synergistic combinations were found.

Document Type: Research Article

Publication date: October 1, 1965

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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