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Chilling vs. Other Methods of Immobilizing Flies

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Insects are often immobilized to facilitate handling during experiments. Of many methods used, one of the earliest was chilling the insects to a point below their activity threshold. As an alternative to chilling, anesthetics such as chloroform and ether have been used. Busvine (1957) pointed out that anesthetics of this type may affect general metabolism and that their fat solvent vapors may disrupt the epicuticular layer of the cuticle. In recent years CO2 has been commonly used. Busvine (1957) has summarized some disadvantages of this method. In this paper we present data on the susceptibility of stable flies, Stomoxys calcitrans (L.), and horn flies, Haematobia irritans (L.), to immobilization by exposure to pure CO2, to a 1:1 mixture of CO2 and air, and to chilling at 4°C.

Document Type: Research Article

Publication date: April 1, 1965

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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