Sensitivity of Bacterial Isolates from Eggs of A phis pomi to Erwinia amyloyora Bacteriophages1, 2
Authors: NEAL, JR., J. W.; ENNS, W. R.; GOODMAN, R. N.
Source: Journal of Economic Entomology, Volume 57, Number 6, December 1964 , pp. 831-832(2)
Publisher: Entomological Society of America
Abstract:Bactrial isolations from eggs of the apple aphid, Aphis pomi Dc Geer, were made during the winters of 196t-62 and 1962-63. In the former, eggs were surface sterilized by immersion in a 10% commercial hypochlorite solution after which aliquots of crushed and uncrushed eggs were cultured in nutrient yeast dextrose broth (NYDB) and then plated on nutrient yeast dextrose agar (NYDA). In the latter, eggs were accumulated on NYDA plates in groups of 20 and strips of the agar medium containing eggs were ground in NYDB, the homogenate incubated for 18 hr at 28°C, and plated on NYDA. Bacteriophage specific for the fireblight organism (Erwinia amylovora) was applied to each plate and in 12 hr read for confluent lysis. A total of 156 bacterial isolates was obtained which could be grouped into 15 categories based on colony morphology. Virulence was ascertained by inoculation into plant tissue. Three categories predominated: white rough; yellow mucoid; and white smooth. Of these, 14 of the 46 white rough isolates were sensitive to the phage but avirulent. None of the yellow mucoids were sensitive although one proved virulent in apple tissue. One white smooth isolate was sensitive and virulent. The remaining isolates were insensitive and avirulent.
Document Type: Research Article
Publication date: 1964-12-01
- Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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