Laboratory Methods for Rearing Rust Mites (Phyllocoptruta oleivora and Aculus pelekassi) on Citrus1

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Abstract:

A method for rearing 2 species of rust mites found on citrus in Florida (the citrus rust mite, Phyllocoptruta oleivora (Ashmead), and Aculus pelekassi Keifer) is described in which large populations were maintained on Murcott Honey orange seedlings during all months of the year. Plants were kept in plastic screen cages in greenhouses at an average temperature of 80 °F. and relative humidity ranging from 30 to 60%. For isolating or confining rust mites within open cages a technique was developed consisting of a ring of lanolin placed on a leaf or fruit.

Mites were transferred by brushing them from an infested plant to a clean plant or fruit with a camel's hair brush, by cutting infested leaves into strips and placing them on a clean leaf or fruit, or by transferring individual mites with a single bristle in a needle holder.

Preliminary investigations showed that the optimum temperature for rearing rust mites was approximately 80 °F, that low humidities were detrimental to rust mites but sustained high humidities increased susceptibility to fungus infection, and that differences in day length affected rust mite populations.

Document Type: Research Article

Publication date: February 1, 1964

More about this publication?
  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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