Diets for Rearing the Sugarcane Borer^1,2

Authors: PAN, YUNG-SONG; LONG, W. H.

Source: Journal of Economic Entomology, Volume 54, Number 2, April 1961 , pp. 257-261(5)

Publisher: Entomological Society of America

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Abstract:

Mass rearing of the sugarcane borer, Diatraea saccharalis, (F.), Was attempted on a limited scale in the laboratory. Larvae were cultured in cotton-stoppered Erlenmeyer flasks containing sugar- cane tops or artificial agar-base diet. Rearing was done under aseptic conditions.

From series of experiments in which the amounts of each ingredient in an artificial diet called the "standard diet" were varied, an "improved diet" was formulated. This improved diet contained corn plant powder(an acetone extract from corn plants, 10.0 grams), sucrose (5 grams), cholesterol (0.2 gram), Wesson's salts (1.0 gram), yeast extract (2 grams), Bacto-agar (3.5 grams), and distilled water (125 milliliters).

Results from several experiments indicated that the optimum amount of sugarcane tops for mass rearing should be 15 grams per insect, and that 7½ grams per insect of the improved artificial diet should be adequate for the complete development of sugarcane borer larvae. Ten insects were reared in each 2.50- milliliter Erlenmeyer flask containing the sugarcane tops. Sugarcane tops appeared to be slightly superior to the improved artificial diet for rearing sugarcane borers.

Document Type: Research article

Publication date: 1961-04-01

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Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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