A Method of Rearing Large Colonies of an Eriophyid Mite, Aceria tulipae (Keifer), in Pure Culture from Single Eggs or Adults1

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Abstract:

A successful method of rearing large colonies of an eriophyid mite, Aceria tulipae (Keifer), vector of wheat streak mosaic virus, from a single mite or egg under greenhouse conditions was devised by using a series of different cages as early colonizing chambers. The cages in the order of their use were (1) petri dish, (2) large test tube plugged with rubber sponge and inverted in a complete nutrient solution, (3) Lamps chimney set in 4-inch flower pot, (4) several types of larger cages of more standard design. Each cage was designed so that eriophyid mites could not gain voluntary or chance entrance or exit. Whole plants bearing mites were transferred from one cage to the next larger one when growth conditions for plants or mites became unfavorable. A high humidity was necessary for rapid increase in mite populations, particularly when colonies were being established. The mites were tea inferred successfully in three ways. Since they proved to be negatively photo tropic, it was possible to herd them by light, either incandescent or flashlight. Mites transferred in this way moved voluntarily and were never injured although it was difficult. Exult to control the number moving. A medium-coarse human hair was the most useful tool for transferring single mites and eggs. The hollow end was used for picking up eggs and the pointed tip for moving nymphs end adults. As the third method portions of mite-infested leaves were inserted into the youngest lea fails of healthy plants end kept in place with a woman's-hair dip. This method was not very practical as mites often did not migrate and died on the excised leaves. Also, it was very difficult to determine the time of migration end the lumber of mites migrating to the new host. High humidity in the micro-environment may be an important factor in the establishment of large mite populations in the field. Although mites thrived Lest in the greenhouse under high humidity conditions, evidence indicated that prolonged flooding (24 hours or more) was detrimental and eventually lethal.

Document Type: Research Article

Publication date: June 1, 1958

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  • Journal of Economic Entomology is published bimonthly in February, April, June, August, October, and December. The journal publishes articles on the economic significance of insects and is divided into the following sections: apiculture & social insects; arthropods in relation to plant disease; forum; insecticide resistance and resistance management; ecotoxicology; biological and microbial control; ecology and behavior; sampling and biostatistics; household and structural insects; medical entomology; molecular entomology; veterinary entomology; forest entomology; horticultural entomology; field and forage crops, and small grains; stored-product; commodity treatment and quarantine entomology; and plant resistance. In addition to research papers, Journal of Economic Entomology publishes Letters to the Editor, interpretive articles in a Forum section, Short Communications, Rapid Communications, and Book Reviews.
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