Authors: Robacker, David C.; Aluja, Martin; Cossé, Allard A.; Sacchetti, Patrizia

Source: Annals of the Entomological Society of America, Volume 102, Number 3, May 2009 , pp. 560-566(7)

Publisher: Entomological Society of America

Abstract:

Attraction of virgin female Anastrepha serpentina Wiedemann (Diptera: Tephritidae) to the odor of calling males was demonstrated. This sex pheromone-mediated attraction occurred during the latter half of a 13-h photophase but not during the first half of the day. Two major components of emissions of calling males, 2,5-dimethylpyrazine (DMP) and 3,6-dihydro-2,5-dimethylpyrazine (DHDMP), and trimethylpyrazine (TMP), a minor component, were tested for pheromonal activity. DMP and TMP elicited antennal responses using electroantennogram assays, but no response was observed for DHDMP. DMP and combinations of DMP and TMP were not attractive in laboratory bioassays. Bioassays of DHDMP were complicated by its instability making purification of the compound unfeasible. Bioassays of DHDMP in unpurified form were further complicated by the presence of reaction byproducts and unreacted reagent in crude preparations of the DHDMP. However, statistical analysis indicated that combinations of 100-500 ng of DHDMP in mixtures with DMP as the major component elicited attraction in laboratory bioassays with sexually active female flies. Taking into account all of the data, we could not conclude that any of the chemicals emitted by males are pheromones, but based on female fly responses to mixtures of DHDMP and DMP, this is the most likely scenario.

Keywords: trimethylpyrazine; 2,5-dimethylpyrazine; dihydropyrazine

Document Type: Research article

Publication date: 2009-05-01

More about this publication?

• Annals of the Entomological Society of America is published in January, March, May, July, September, and November. Annals especially invites submission of manuscripts that integrate different areas of insect biology, and address issues that are likely to be of broad relevance to entomologists. Articles also report on basic aspects of the biology of arthropods, divided into categories by subject matter: systematics; ecology and population biology; arthropod biology; arthropods in relation to plant diseases; conservation biology and biodiversity; physiology, biochemistry, and toxicology; morphology, histology, and fine structure; genetics; and behavior.
• Editorial Board
• Submit a Paper
• Subscribe to this Title
• Information for Advertisers
• Visit this journal's homepage
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Entomology
• By this author: Robacker, David C. ;  Aluja, Martin ;  Cossé, Allard A. ;  Sacchetti, Patrizia

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers