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Open Access Evaluación de medios de cultivo para la producción in vitro de Annona muricata mediante la técnica de microinjertación seriada Evaluation of culture media for in vitro production of Annona muricata using the serial grafting technique

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Annona muricata (guanábana) es una planta cultivada por la calidad nutritiva y organoléptica de su fruto. No obstante el carácter recalcitrante de las yemas vegetativas de esta especie ha dificultado la micropropagación de material seleccionado por su alta productividad. Esta investigación tuvo como objetivo evaluar medios de cultivo combinados con la técnica de microinjertación seriada para promover el desarrollo vegetal in vitro de A. muricata. En medios de cultivo específicos para cada material vegetal, se sembraron semillas y estacas para la formación de microinjertos&59; luego se aplicaron tres tratamientos de microinjertación (M1, M2, M3) y un control, donde el número indica las veces en las que una estaca es injertada en un portainjerto. M1 es una microinjertación monofásica o estacas microinjertadas en un portainjerto. M2 son estacas microinjertadas dos veces, es decir, permanecen en dos portainjertos diferentes con un lapso de un mes entre cada traspaso. M3 son estacas microinjertadas tres veces, es decir, permanecen en tres portainjertos diferentes con un lapso de un mes entre cada traspaso. Las estacas fueron separadas del portainjerto y sembradas en medio de cultivo para evaluar su revigorización&59; se realizaron pruebas de Anova y Freedman para los datos paramétricos y no paramétricos, respectivamente. Los resultados indicaron que la microinjertación monofásica es suficiente para estimular el desarrollo in vitro de material adulto de A. muricata&59; sin embargo, en los tratamientos no se logró el desarrollo de un sistema radicular adecuado.&60;br&62;Annona muricata is grown due to the nutritional and organoleptic quality of the fruit. The recalcitrance of the vegetative buds of this species has hindered the micropropagation of selected material according to its high productivity. This research aimed to evaluate culture medium combined with the technique of serial micrografting to promote in vitro plant growth of Annona muricata. In specific culture media for each plant material, seeds and cuttings were planted for the formation of micrografts. Three micrografting treatments were applied (M1, M2, M3) and a control, where the number indicates the times when a shoot is micrografted onto a rootstock. M1 is a monophasic micrografting or cuttings micrografted in one rootstock. M2 are micrografted cuttings for two times, that is, these remain on two different rootstocks with a lapse of one month between each transfer. M3 are micrografted cuttings three times, that is, these remain in three different rootstocks with a lapse of one month between each transfer. The shoots were separated from the rootstock and planted in culture medium to assess their reinvigoration, ANOVA and Freedman tests were performed for parametric and nonparametric data respectively. The results indicated that the monophasic micrografting is sufficient to stimulate the in vitro development of adult material of Annona muricata&59; however, none of the treatments was able to develop a root system in the shoots.
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Document Type: Research Article

Publication date: 01 January 2011

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