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Open Access Propagación in vitro de Heliconia bihai (L.) cv. Lobster Salmón a partir de meristemos florales In vitro propagation of Heliconia bihai (L.) cv. Lobster Salmón from flowering buds

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Se desarrolló un protocolo de propagación in vitro a partir de meristemos florales para Heliconia bihai cv. Lobster Salmón. Para el efecto se establecieron 250 yemas florales en diferentes tiempos utilizando NaClO al 1% durante 20 min. El medio de establecimiento Murashige y Skoog con mejores resultados fue BAP (2 mg/lt), AIA (1 mg/lt) y 0.1 g/lt de L-cisteína. La mayor produccion de brotes en multiplicación se obtuvo con 6 mg/lt de BAP y con un subcultivo posterior en medio con 2 mg/lt de BAP y 0.5 mg/lt de AIA, alcanzando una tasa de multiplicación de tres nuevas yemas. El enraizamiento de las plántulas in vitro se logró con 1.3 mg/lt de AIA. La aclimatación en vivero fue 92% de supervivencia. Se realizó un análisis de varianza (Anova) y un test de Tukey para las variables evaluadas las cuales mostraron diferencias estadísticas en cada fase. La propagación in vitro de heliconias a partir de yemas florales requiere mayor tiempo en la fase de establecimiento y multiplicación, no obstante permite mayores porcentajes de supervivencia y una menor contaminación, ofreciendo material de siembra de diferentes especies de esta planta de interés comercial.&60;br&62;A protocol for in vitro propagation of Heliconia bihai cv. Lobster Salmón was developed using flowering buds as initial explants. 250 flowering buds were established in different assays with NaClO at 1% during 20 minutes. The establishment in Murashige & Skoog's medium with BAP (2mg/L), AIA (1 mg/L) and L-cystein (0.1 g/L) showed the best results. Shoot multiplication was obtained with BAP (6 mg/L) with a subsequent subculture in a medium with BAP (2 mg/L) and AIA (0.5 mg/L), reaching multiplication rates of 3.0 new shoots. Rooting was achieved with AIA 1.3 mg/L. The survival rate during greenhouse acclimatization was 92%. Analysis of variance (ANOVA) and Turkey's test were realized for each of the variables evaluated that showed statistical differences in all stages. The in vitro propagation of heliconia with flowering buds, requires longer periods for establishment and multiplication stages but allows higher levels of survival for initial explants and lower contamination, offering an alternative to obtain important amounts of planting material of different heliconia species of commercial interest.
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Document Type: Research Article

Publication date: 01 January 2011

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