The effect of ectomycorrhizal fungi and bacteria on pine seedlings

The effect of ecomycorrhizal fungi (Hebelon crustuliniforme(Bull.&58; Fr.) Quél. 5392 and Pisolithus tinctorius (Pers.) Coker et Couch 5335) and bacteria (Bacillus polymyxa and Azospirillum brasilense). associated with mycorrhizas on the growth of pine seedligs was investigated. In addition the influence of bacteria on fungal biomass production and the relationship between ectomycorrhizal fungi and fungi pathogenic to root of pine seedlings were determined. In general, the shoot/root ratio was higher in plants inoculated with Hebeloma crustuliniforme and bacteria than in the control seedlings (grown only under sterile conditions). In non-sterile substrate the root/shoot ratio of the mycorrhizal seedlings was lower as compared to the control. Similar phenomenon was noted in plants inoculated with the mycorrhizal fungus Pisolithus tinetorius. The bacteria used as well as the time of introduction of these organisms into the cultures of mycorrhiza fungi affected the production of fungal biomass. Hebeloma crustuliniforme and Pisolithus tinctorius inhibited the growth of Rizoctonia solani and Fusarium oxysporum fungi pathogenic to pine seedlings.