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Free Content Nuclear Status and Dna Fragmentation of Oocytes From Porcine, Bovine and Feline Ovaries Stored at 4°C for 5 Days

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BACKGROUND: The cooling of mammalian oocytes to sub-physiological temperatures is widely known to affect their viability through the induction of various abnormalities at all stages of meiosis. OBJECTIVE: This study was to compare the kinetics of nuclear status and oocyte damage in porcine, bovine and feline ovaries stored at 4°C for 5 days. METHODS: The nuclear status and oocyte quality during storage were evaluated before and after maturation culture. RESULTS: The cold storage of ovaries decreased the proportions of porcine and bovine oocytes that remained at the germinal vesicle stage before maturation culture. The maturation rates of oocytes decreased with increasing storage time, independent of species. None of the porcine oocytes reached metaphase II (MII) after 1 day of storage. In contrast, bovine and feline oocytes from ovaries that were stored for 2 days and 3 days reached MII. DNA fragmentation in porcine oocytes from ovaries stored for 1 day was significantly higher than that in bovine and feline oocytes. CONCLUSION: The maturation competency of oocytes after the cold storage of ovaries could be related to the meiotic resumption of oocytes during storage and the occurrence of DNA fragmentation in oocytes during maturation culture.

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Keywords: CHILLING SENSITIVITY; DNA DAMAGE; LONG-TERM STORAGE; MEIOTIC COMPETENCY

Document Type: Research Article

Publication date: 01 January 2014

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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