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Free Content Human Spermatozoa Cryopreservation: Comparison of Three Different Freezing Protocols

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Human spermatozoa cryopreservation has significantly improved over the last few decades, but the actual protocols are neither optimal nor standardized between different laboratories in spite of the importance of preserving male fertility or treating severely infertile males.

In the present study we aimed to determine the best in-house method of rapid freezing in terms of sperm motility and vitality by comparing three different rapid methods of human spermatozoa cryopreservation.

Our data showed that M1 (triphasic cooling) is the method associated with a significantly lower deterioration of semen quality in comparison with mono or biphasic cooling. Differences observed among the three protocols were supported by statistical analysis.

These data reinforce previous evidences about the influence of sperm quality on IVF outcome and suggest the importance of improving sperm cryopreservation techniques especially when semen is seriously compromised at baseline.
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Keywords: FERTILITY PRESERVATION; FREEZING PROTOCOL; HUMAN SPERMATOZOA CRYOPRESERVATION; SPERM MOTILITY; SPERM VIABILITY

Document Type: Research Article

Publication date: 2013-09-01

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  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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