Seminal Freezing in Pure Breed Andalusian Horse: Difference in Individual Stallions and Correlation Between Pre and Post-Freezing Sperm Parameters
Abstract:The aim of this study was the optimization of the sperm freezing protocols for the Pure Breed Andalusian Horse (AH) stallions. The study was performed in 84 ejaculates from 14 stallions (6 ejaculates per stallion). We examined the effect of individual stallion, centrifugal force and centrifugation extender on post-thaw sperm quality. Neither centrifugal force nor centrifugal extender had any significant effect on post-centrifugation or post-thawing sperm quality. Stallion was the principal source of variation in our experiments, showing individual significant differences (p<0.05) in all parameters. Individual differences were more extreme prior to freezing than after freezing-thawing. There are significant positive correlations (p<0.05 and p<0.01) between all post-centrifugation and post-thawing parameters.
Document Type: Research Article
Publication date: November 1, 2011
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.