Apoptosis in Human Ovarian Tissue after Conventional Freezing or Vitrification and Xenotransplantation

Authors: Rahimi, Gohar; Isachenko, Vladimir; Todorov, Plamen; Tawadros, Samir; Mallmann, Peter; Nawroth, Frank; Isachenko, Evgenia

Source: Cryoletters, Volume 30, Number 4, July 2009 , pp. 300-309(10)

Publisher: Cryoletters

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Abstract:

One of the new emerging techniques to preserve reproductive potential of cancer patients is cryopreservation of ovarian fragments prior to medical treatment and their retransplantation after healing. In order to investigate and compare apoptosis in human ovarian tissue after conventional ("slow") freezing and vitrification, we used a xenograft model in which conventionally frozen, vitrified and fresh non treated human ovarian tissue pieces were subcutaneously transplanted in SCID mice. The tissue samples were weekly, during four weeks, recovered from scarified SCID mice. The apoptosis was examined by immunohistochemical staining with the anti-caspase-3 antibody. There was a significant difference between the amount of apoptotic cells in cryopreserved ovarian tissue independent from mode of cooling compare to the control. The ovarian tissue after vitrification showed a significantly higher amount of apoptotic cells, than in slow frozen. The results obtained after comparative study of two different cryopreservation methods show that vitrification of human ovarian tissue could become a practice-relevant alternative to slow cryopreservation only after further improvement.

Keywords: APOPTOSIS; HUMAN OVARIAN TISSUE; SLOW FREEZING; VITRIFICATION; TRANSPLANTATION

Document Type: Research article

Publication date: 2009-07-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Apoptosis in Human Ovarian Tissue after Conventional Freezing or Vitrification and Xenotransplantation

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