Cryopreservation of Ginkgo Biloba Cell Culture: Effect of Pretreatment with Sucrose and Aba

Authors: Lu, Zhi-Wei; Popova, Elena V.; Wu, Chun-Hua; Lee, Eun-Jung; Hahn, Eun-Joo; Paek, Kee-Yoeup

Source: Cryoletters, Volume 30, Number 3, May 2009 , pp. 232-243(12)

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Abstract:

The report describes the impact of preculture with sucrose and sucrose + ABA on desiccation and cryopreservation tolerance of cell cultures of Ginkgo biloba L., an important landscape and medicinal tree. Callus clumps were incubated on MS medium supplemented with high sucrose concentrations (up to 24%, w/v), employed alone or with ABA (2-10 mg l⁻¹) for various durations followed by desiccation for 0-240 min and cryopreservation. The beneficial effect of preculture on regrowth after desiccation without cryopreservation was only observed for the cells with water content of 20% FW and was not influenced by presence of ABA. However, preculture of calli in presence of ABA resulted in a lower desiccation rate as compared with untreated controls and calli pretreated with sucrose alone. In calli precultured with sucrose alone, post-thaw regrowth was occasional regardless of the sugar concentration in the medium, while pretreatment of calli with ABA and sucrose ensured stable regrowth after cryopreservation. The highest post-thaw regrowth of 54% was achieved for calli precultured on medium supplemented with 10% (w/v) sucrose and 2 mg l⁻¹ ABA for 21 days followed by desiccation for 150 min. The different effects of preculture treatments on post-thaw regrowth were associated with significant changes in content and in composition of endogenous soluble sugars in calli. Sucrose and glucose accumulated preferentially in ABA-precultured calli, while the fructose content was higher in calli precultured in absence of ABA. The possible role of preculture on desiccation and cryopreservation tolerance of G. biloba cell cultures is discussed.

Keywords: GINKGO BILOBA; CALLUS; CRYOPRESERVATION; ABSCISIC ACID; SUCROSE PRECULTURE; SOLUBLE SUGAR CONTENT

Document Type: Research article

Publication date: 2009-05-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Cryopreservation of Ginkgo Biloba Cell Culture: Effect of Pretreatment with Sucrose and Aba

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