Cryopreservation by Encapsulation-Dehydration of Plumules of Coconut (Cocos nucifera L.)

Authors: N'Nan, Oulo; Hocher, Valérie; Verdeil, Jean-Luc; Konan, Jean-Louis; Ballo, Koffi; Mondeil, Fanja; Malaurie, Bernard

Source: Cryoletters, Volume 29, Number 4, July/August 2008 , pp. 339-350(12)

Publisher: Cryoletters

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Abstract:

This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20% which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.

Keywords: COCOS NUCIFERA L; CRYOPRESERVATION; ENCAPSULATION-DEHYDRATION; HISTOLOGY; PLUMULE; SUCROSE

Document Type: Regular paper

Publication date: 2008-07-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.