Cryopreservation of Embryonic Axes of Selected Amaryllid Species
Abstract:A study on cryopreservation of excised embryonic axes of fifteen species of the Amaryllidaceae is reported. Embryonic axes that after flash-drying had a water content in the range 0.4 to 0.1 g g-1 and survival ≥60% were selected for cryopreservation procedures. The highest post-thaw viabilities (roots and shoots produced) across all species were recorded for embryonic axes subjected to rapid rather than slow cooling. With rapid cooling and no cryoprotection, the highest post-thaw viabilities for the fifteen species investigated was 0% in one species; ranged between 10 and 35% for nine species; and between 45 and 55% for five species. With cryoprotection and rapid cooling the highest post-thaw viabilities for these fifteen species was 0% for one species; ranged between 15 and 35% for six species; and between 40 and 75% for eight species. The highest post-thaw survival in ten out of fifteen species was obtained for axes dried to between 0.24±0.06 and 0.14±0.08 g g-1 (and rapidly cooled). With only one exception (Strumaria discifera; 45%), post-thaw survival after slow cooling ranged between 10 and 30%. Survival after vitrification plus slow cooling was achieved for seven species but was never higher than post-thaw survival in non-cryoprotected, rapidly cooled axes. The results suggest that species within the same family can exhibit commonalities in terms of amenability to cryopreservation techniques but for maximum success, axis water content and cooling rate particularly, must be optimised for each species in the family independently.
Document Type: Research Article
Publication date: September 1, 2007
CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.