Free Content Field Development of Oil Palms (Elœis guineensis Jacq.) Originating from Cryopreserved Stabilized Polyembryonic Cultures

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Abstract:

In this paper, the long term observation of plants originating from control and cryopreserved stabilized polyembryonic cultures (SPCs) of six elite oil palm clones was carried out. Survival of plantlets in the nursery was monitored, then a series of vegetative and floral characteristics of over 440 palms were studied for up to 12 years after field transfer in Côte d'Ivoire. The six clones tested showed an average recovery of 34% after freezing in liquid nitrogen. The average survival in the nursery of plantlets originating from pretreated and dehydrated and from cryopreserved SPCs was higher than that of control SPCs. Palm trees originating from control SPCs were found to flower earlier than those originating from pretreated and dehydrated and from cryopreserved SPCs. This delay in flowering disappeared progressively and all palms had flowered 3 years after planting regardless of the SPC treatment. Abnormal palms were observed in one clone only. With this clone, the percentage of abnormal palms originating from cryopreserved SPCs was significantly lower (5%) than that measured on palms originating from control SPCs (29%).

Keywords: CRYOPRESERVATION; FIELD PLANT DEVELOPMENT; FLOWERING; OIL PALM; STABILIZED POLYEMBRYONIC CULTURES

Document Type: Research Article

Publication date: September 1, 2007

More about this publication?
  • CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

    The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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