Effects of Removalof Necrotic Blastomeres from Human Cryopreserved Embryos on Pregnancy Outcome

Authors: Liu, Wei-Xin; Luo, Meng-Jun; Huang, Ping; Wang, Li; Zhao, Cheng-Yuan; Yue, Li-Min; Zheng, Yu

Source: Cryoletters, Volume 28, Number 2, March/April 2007 , pp. 129-136(8)

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Abstract:

This study assessed whether the implantation potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres. We retrospectively analyzed the pregnancy rate and implantation rate of 170 human frozen embryo transfer cycles. Laser-assisted hatching and micromanipulation were performed to remove the necrotic blastomeres. A higher clinical pregnancy rate (22.22%) and embryo implantation rate (10.17%) were observed when transferred embryos comprised fully intact and partially damaged embryos compared with partially damaged embryos alone (5.88% and 2.82%, respectively). When transferred embryos were fully intact and partially damaged embryos, removal of necrotic blastomeres from partially damaged embryos significantly increased the clinical pregnancy rate (43.90% versus 24.00%, P<0.05) and the implantation rate (19.44% versus 10.29%, P<0.05). The results indicated that the implantation potential of partially damaged cryopreserved embryos can be improved by removal of necrotic blastomeres with laser-assisted hatching and micromanipulation.

Keywords: HUMAN EMBRYO; CRYOPRESERVATION; NECROTIC BLASTOMERES; IMPLANTATION POTENTIAL

Document Type: Research article

Publication date: 2007-03-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Effects of Removalof Necrotic Blastomeres from Human Cryopreserved Embryos on Pregnancy Outcome

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