Isolation and some characterisation of antifreeze protein from the european eelpout Zoarces viviparus

Authors: Sørensen, T.F.; Cheng, C-H.C.; Ramløv, H.

Source: Cryoletters, Volume 27, Number 6, November/December 2006 , pp. 387-399(13)

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Abstract:

The European eelpout Zoarces viviparus is a common inhabitant in the coastal areas of the eastern Atlantic Ocean and the Baltic region. At least 3 different antifreeze proteins were purified from Z. viviparus serum but more isoforms are most likely present. Two antifreeze proteins with molecular weights of approx. 6.5-7 kDa were characterised and found to share high similarity to the type III antifreeze proteins found in other members of the family Zoarcidae. The antifreeze activity of Z. viviparus antifreeze proteins is concentration dependent and showed a saturation effect when the protein concentration reached 30mg·ml⁻¹ (crude serum) and 8mg·ml⁻¹ (partly purified serum) respectively. Further the antifreeze activity was found to be dependent of the buffer osmolality resulting in increasing thermal hysteresis when buffer osmolality was raised from 0 to 1M.

Keywords: ANTIFREEZE PROTEINS; ZOARCES VIVIPARUS; ZOARCIDAE; PURIFICATION; CHARACTERISATION

Document Type: Research article

Publication date: 2006-11-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.