Vitrification of caudal fin explants from zebrafish adult specimens

Authors: Cardona-Costa, J.; Roig, J.; Perez-Camps, M.; García-Ximénez, F.

Source: Cryoletters, Volume 27, Number 5, September/October 2006 , pp. 329-332(4)

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Abstract:

No data on vitrification of tissue samples are available in fishes. Three vitrification solutions were compared: V1: 20% ethylene glycol and 20% dimethyl sulphoxide; V2: 25% propylene glycol and 20% dimethyl sulphoxide, and; V3: 20% propylene glycol and 13% methanol, all three prepared in Hanks' buffered salt solution plus 20% FBS, following the same one step vitrification procedure developed in mammals. Caudal fin tissue pieces were vitrified into 0.25 ml plastic straws in 30s and stored in liquid nitrogen for 3 days minimum, warmed (10s in nitrogen vapour and 5s in a 25°C water bath) and cultured (L-15 plus 20% FBS at 28.5°C). At the third day of culture, both attachment and outgrowing rates were recorded. V3 led to the worst results (8% of attachment rate). V1 and V2 allow higher attachment rates (V1: 63% vs V2: 50%. P < 0.05) but not significantly different outgrowing rates (83%-94%). Vitrification of caudal fin pieces is advantageous in fish biodiversity conservation, particularly in the wild, due to the simplicity of procedure and equipment.

Keywords: CRYOPRESERVATION; VITRIFICATION; CRYOPROTECTANTS; ZEBRAFISH; TISSUE CULTURE; BIODIVERSITY

Document Type: Research article

Publication date: 2006-09-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Vitrification of caudal fin explants from zebrafish adult specimens

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