Cryopreservation of rabbit spermatozoa with freezing media supplemented with reduced and oxidised glutathione

Authors: Marco-Jiménez, F.; Lavara, R.; Vicente, J.S.; Viudes-de-Castro, M.P.

Source: Cryoletters, Volume 27, Number 4, June 2006 , pp. 261-268(8)

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Abstract:

This study researches the effects of supplementation with reduced glutathione (GSH, 0.5 mM) and oxidised glutathione (GSSG, 0.5 mM) freezing extenders on different semen parameters after equilibration with DMSO preservation solution (45 min at 5° C) and post-thawing. The main findings that emerged from this study are that (i) addition of GSH and GSSG to the freezing media did not result in any improvement in functional sperm tests after equilibrium phase. (ii) No differences were observed after cryopreservation in functional sperm tests and embryo recovery rate. In conclusion, the addition of 0.5 mM GSH or GSSG appears not to play an important role in sperm antioxidant defence during cooling and freezing in rabbit spermatozoa.

Keywords: SPERM; RABBIT; GLUTATHIONE; CRYOPRESERVATION; FLOW CYTOMETRY

Document Type: Research article

Publication date: 2006-06-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.