Hypothermic renal preservation with a sucrose/ polyethylene glycol solution in a rabbit renal transplant model

Authors: Fuller, Barry J.; Shurey, Colin; Lane, Nick; Petrenko, Alex; Green, Colin

Source: Cryoletters, Volume 27, Number 2, March/April 2006 , pp. 127-132(6)

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Abstract:

Renal preservation at for 24 hours at hypothermia was studied in a rabbit model after flush cooling with sucrose-based solution (SBS), compared with a standard preservation solution (in this case, Marshall's Hypertonic Citrate solution - HCA). Polyethylene glycol supplementation to SBS (SBS-PEG) was also investigated. Renal function was measured by plasma creatinine assays during 1 months post transplantation, and pathology of the explanted kidneys was undertaken. Results showed that survival at 28 days was similar in all groups, (HCA – 3/6; SBS – 2/5; SBS-PEG – 3/5), and there were no differences in recovery of plasma creatinine values. Histopathological evaluation of the grafts indicated that SBS preservation resulted in more severe damage after transplantation (P<0.05 in both cortico-medullary region and medulla compared to HCA), whilst addition of PEG reduced the damage score to that seen with HCA. SBS can be used as a simple, inexpensive preservation solution for kidney cold storage provided that PEG is used as an additional colloid.

Keywords: KIDNEY; RENAL COLD PRESERVATION; SUCROSE-BASED SOLUTION; POLYETHYLENE GLYCOL; KIDNEY TRANSPLANTATION; RABBIT

Document Type: Research article

Publication date: 2006-03-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Hypothermic renal preservation with a sucrose/ polyethylene glycol solution in a rabbit renal transplant model

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