Ultrastructural implications of pretreatment for successful cryopreservation of oncidium protocorm-like body

Authors: Miao, Nae-Hwey; Kaneko, Yasuko; Sugawara, Yasutake

Source: Cryoletters, Volume 26, Number 5, September 2005 , pp. 333-340(8)

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Abstract:

By applying pre-treatment with high concentrations of sucrose and glycerol prior to desiccation and subsequent freezing in liquid nitrogen, successful cryopreservation with high recovery rate was achieved in Oncidium PLB(protocorm-like body). Cellular and subcellular changes after each step in various cryopreservation regimes were examined to elucidate the mechanism of the beneficial effect of the pretreatment which confers resistance to desiccation and freezing.

Keywords: CRYOPRESERVATION; DESICCATION AND FREEZING INJURY; ELECTRON MICROSCOPIC OBSERVATION; ONCIDIUM HAMANA "ELFIN"; PROTOCORM-LIKE BODY (PLB)

Document Type: Regular paper

Publication date: 2005-09-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.