Integrity of endothelium in cryopreserved human cornea

Authors: Neronov, A.; Mazgalova, J.; Cholakova, M.; Dimitrova, M.; Deligiozova, I.; Kovatcheva, S.; Nikolova, E.

Source: Cryoletters, Volume 26, Number 2, March/April 2005 , pp. 131-136(6)

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Abstract:

The aim of the present study was to elaborate an optimal method for cryopreservation of human donor cornea for transplantation and to follow the morphological changes in the structure of the endothelial cell layer using scanning electron microscopy (SEM). Sixteen groups, with four donor cornea each, were cryopreserved at cooling rates of 1°/min and 5°/min. Four cryoprotectants (glycerol, dimethyl sulfoxide, 1,2-propanediol, polyethylene glycol-400) in two concentrations - 5% and 10 % v/v, were prepared on the bases of medium Optisol GS supplied with 20 % v/v human serum albumin. Four additional human cornea were used as controls. Endothelial cell recovery of the cornea after thawing and 24 hours culture, was calculated as a percent of the preserved recovered cells. Sufficient recovery of the endothelial cell layer, making the cornea suitable for transplantation was obtained using the cryoprotectants dimethyl sulfoxide and especially polyethylene glycol-400.

Keywords: HUMAN CORNEA; CRYOPRESERVATION; SEM OBSERVATION

Document Type: Regular paper

Publication date: 2005-03-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.