Experimental Determination of Human Erythrocyte Membrane Permeability Coefficients for a Series of Amides

Authors: Gordiyenko Olga I.; Gordiyenko Yuliya E.; Linnik Tamara P.; Gordiyenko Evgen O.

Source: Cryoletters, Volume 26, Number 1, January 2005 , pp. 65-72(8)

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Abstract:

Erythrocyte membrane permeability coefficients have been determined for a series of amides by a method based on the physical and mathematical modelling of hypotonic haemolysis process. The results show that penetration of the substances occurs by two alternative ways – through aqueous pores formed by proteins and by the direct dissolving of the molecules in membrane lipids. This conclusion can be confirmed by the correlation analysis between permeability coefficients of native erythrocytes and those pre-incubated with the monosodium salt of p-chloromercuribenzenesulfonic acid (pCMBS), and the partition coefficients of the substances in hydrophilic-hydrophobic phases. Penetration of substances through hydrophilic channels is limited by the sterical factor and diameter in particular. Permeability coefficients for erythrocytes pre-incubated with pCMBS increase in an accordance with the rise of the partition coefficients with correlation coefficient of 0.94, thereby indicating a lipid route of permeation of molecules through erythrocyte membranes.

Keywords: HUMAN ERYTHROCYTE; PERMEABILITY COEFFICIENTS; AMIDES

Document Type: Regular paper

Publication date: 2005-01-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

Experimental Determination of Human Erythrocyte Membrane Permeability Coefficients for a Series of Amides

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