Effect of the extender supplement equex-stm on cryopreserved semen in the Assaf sheep

Authors: Akourki A.; Gil L.; Echegaray A.; Espinosa E.; Josa A.; De Blas I.; Gonzalez N.; Gallegos de la Hoya M.; Meque L.C.

Source: Cryoletters, Volume 25, Number 2, 1 February 2004 , pp. 147-154(8)

Publisher: Cryoletters

Abstract:

This study was designed to evaluate the effect of adding the detergent Equex-STM to the extender used to dilute semen for cryopreservation on several indicators of sperm preservation. Two consecutive ejaculates per day were obtained from 5 Assaf sheep on two days out of every week over three alternate months. The freezing protocol involved diluting the semen in Fiser's extender, to which 0.7 % Equex-STM was added or omitted before cryopreserving the semen in straws by exposure to nitrogen vapor. Equex-STM supplementation gave rise to significantly (p<0.05) improved sperm quality variables after different periods of freezing (0 hours, 1 week and 1 month). The variables examined were: individual motility, viability, acrosome integrity, plasma membrane integrity (HOS test) and morphological anomalies. This improvement was independent of the ram and month of testing. In a second experiment in which we incubated the semen (0 and 6 hours) at 37°C after thawing, Equex-STM also showed a beneficial effect on sperm quality.

Keywords: ASSAF SHEEP; SEMEN; EQUEX-STM; EXTENDER; CRYOPRESERVATION

Document Type: Research article

Publication date: 2004-02-01

More about this publication?

• CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation

  The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.

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