Non-equilibrium cooling of Poncirus trifoliata (L.) embryonic axes at various water contents

Authors: Wesley-Smith J.; Walters C.; Berjak P.; Pammenter N.

Source: Cryoletters, Volume 25, Number 2, 1 February 2004 , pp. 121-128(8)

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Abstract:

The present study investigated the rate of temperature change within axes of Poncirus trifoliata during cooling and warming by various methods. Cooling rates ranged between 0.17 and 1700°C s^-1, and warming rates of 1.25 and 600°C s^-1 were measured when axes were warmed at room temperature or in water at 40°C, respectively. Partial drying increased the cooling rate within axes in direct contact with the cryogen, but did not affect the cooling or warming rates within axes enclosed in a double layer of lightweight aluminium foil. The procedures described illustrate the orders of magnitude that separate extremes of the range of cooling or warming rates attained using methods commonly employed in cryopreservation studies. Quantifying these rates allows the relationship between cooling rate, water content and survival of hydrated embryonic axes to be explored.

Keywords: EMBRYONIC AXES; RAPID COOLING; DEHYDRATION; PONCIRUS TRIFOLIATA; WARMING; RECALCITRANCE; CRYOPRESERVATION

Document Type: Research article

Publication date: 2004-02-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.