A comparision of four methods for cryopreservation of meristems in banana (Musa spp.)

Authors: Agrawal A.; Swennen R.; Panis B.

Source: Cryoletters, Volume 25, Number 2, 1 February 2004 , pp. 101-110(10)

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Abstract:

To establish an improved protocol for the cryopreservation of banana (Musa spp.), a fast-freeze/fast-thaw method was applied and compared to three existing cryogenic procedures, namely, simple freezing, vitrification of proliferating meristems and vitrification of individual meristems. The average post-thaw shoot regeneration of the fast freeze/fast thaw protocol was 51.8% and was superior to the existing protocols which were 12.5%, 39.2% and 42.8%, respectively. Other factors such as time and ease of operation were considered for each method. It was found that the fast freeze/fast thaw technique was the best amongst all the four methods tested for the cryopreservation of in vitro banana meristems in the genotype Robusta (AAA).

Keywords: BANANA; BENZYLADENINE; CRYOPRESERVATION; CRYOPROTECTION; MERISTEM; MUSA SPP; ROBUSTA; SUCROSE; VITRIFICATION

Document Type: Research article

Publication date: 2004-02-01

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CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation
The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.