A comparision of four methods for cryopreservation of meristems in banana (Musa spp.)

Authors: Agrawal A.; Swennen R.; Panis B.

Source: Cryoletters, Volume 25, Number 2, 1 February 2004 , pp. 101-110(10)

Publisher: Cryoletters

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Abstract:

To establish an improved protocol for the cryopreservation of banana (Musa spp.), a fast-freeze/fast-thaw method was applied and compared to three existing cryogenic procedures, namely, simple freezing, vitrification of proliferating meristems and vitrification of individual meristems. The average post-thaw shoot regeneration of the fast freeze/fast thaw protocol was 51.8% and was superior to the existing protocols which were 12.5%, 39.2% and 42.8%, respectively. Other factors such as time and ease of operation were considered for each method. It was found that the fast freeze/fast thaw technique was the best amongst all the four methods tested for the cryopreservation of in vitro banana meristems in the genotype Robusta (AAA).

Keywords: BANANA; BENZYLADENINE; CRYOPRESERVATION; CRYOPROTECTION; MERISTEM; MUSA SPP; ROBUSTA; SUCROSE; VITRIFICATION

Document Type: Research article

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