@article {Burch:2002:0143-2044:197,
title = "Cryopreservation of protonemata of Ditrichum cornubicum (Paton) comparing the effectiveness of four cryoprotectant pretreatments",
journal = "Cryoletters",
parent_itemid = "infobike://cryo/cryo",
publishercode ="cryo",
year = "2002",
volume = "23",
number = "3",
publication date ="2002-05-01T00:00:00",
pages = "197-208",
itemtype = "ARTICLE",
issn = "0143-2044",
eissn = "1742-0644",
url = "https://www.ingentaconnect.com/content/cryo/cryo/2002/00000023/00000003/art00011",
keyword = "CRYOPRESERVATION, BRYOPHYTE, SUCROSE, EX SITU CONSERVATION, ABA",
author = "Burch, J. and Wilkinson, T.",
abstract = "If an ex situ programme for the conservation of rare and threatened bryophytes is to succeed, a method of long-term, basal storage is required. The aim of this study was to determine the effectiveness of sucrose, ABA and sucrose/ABA combined, as cryoprotectant pretreatments, for the long-term storage of protonemata of the endangered Cornish path moss, Ditrichum cornubicum. This work is a prelude to the in vitro conservation of rare and endangered UK bryophytes. The addition of sucrose and/or ABA in the pre-culture medium, reduced protonemal growth rate. However, pretreatment afforded a high level of protection against tissue damage during dehydration and freezing, with 100 % regeneration of pretreated plants after thawing, compared to 53 % regeneration of controls. Sucrose combined with ABA in the pre-culture medium gave the most effective cryoprotection, with protonemata having fewer necrotic tissues and rapid recovery of growth.",
}