Knockdown of Bmi-1 Impairs Growth and Invasiveness of Human Gastric Carcinoma Cells
The effect of B cell-specific MLV integration site-1 (Bmi-1) RNA interference (RNAi)-mediated inhibition of Bmi-1 expression on the proliferation, apoptosis, and invasiveness of human gastric carcinoma AGS cells was investigated. RT-PCR and Western blot analyses demonstrated that Bmi-1 expression and the Bmi-1 protein level were significantly decreased in Bmi-1 shRNA transfected AGS cells compared to untransfected and nonspecific shRNA transfected AGS cells. Bmi-1 RNAi-mediated inhibition of Bmi-1 expression significantly affected cell growth and invasiveness, and resulted in increased AGS cell apoptosis. This was not observed in untransfected and nonspecific shRNA transfected AGS cells. Inhibition of Bmi-1 expression in human gastric carcinoma cells affects cell proliferation and invasiveness.
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Document Type: Research Article
Publication date: 2009-11-01
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- Formerly: Oncology Research Incorporating Anti-Cancer Drug Design
Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.