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Hepal-6 Derived RNA Electroporated Murine Spleen B Cells Induced Antitumor Effects In Vivo

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Abstract:

RNA electroporated CD40 ligand-activated B cells can induce cytotoxic T-lymphocyte response in vitro. In order to evaluate the effects in vivo, we applied murine spleen B cell vaccine in a mouse model of liver cancer. C57BL/6 mouse spleen B cells were activated by anti-mouse CD40 antibody, recombinant interleukin-4, and cyclosporin A, and then electroporated with total RNA from Hepal-6 cells (Hepal-6 RNA-CD40mAb-B cells). This vaccine was injected into C57BL/6 mice that were subcutaneously inoculated with Hepal-6 cells. Hepal-6 RNA-CD40mAb-B cells could induce tumor-specific cytotoxic T cells and IFN-γ secretion in the immunized mice. In vivo study showed this vaccine could well inhibit tumor progression, improve overall survival, and provide continuous immunoprotection against Hepal-6 cell-induced tumor. Tumor cell-derived total RNA electroporated B cells vaccine is a type of effective immunotherapy and provides potential implication for clinical treatment.

Keywords: B cell; C57BL/6 mouse; CD40; Hepatocellular carcinoma; Immunotherapy

Document Type: Research Article

DOI: http://dx.doi.org/10.3727/096504009789735468

Publication date: January 1, 2009

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  • Formerly: Oncology Research Incorporating Anti-Cancer Drug Design
    Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.
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