Authors: Grau, Elena¹; Oltra, Silvestre¹; Orellana, Carmen¹; Hernández-Martí, Miguel²; Castel, Victoria³; Martínez, and Francisco¹

Source: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Volume 15, Numbers 7-8, 2005 , pp. 393-398(6)

Publisher: Cognizant Communication Corporation

Abstract:

Neuroblastoma and pheochromocytoma have the same embryonal origin. They originate from neural crest cells, and they usually affect suprarenal glands. The SDHB gene encodes the B subunit of succinate dehydrogenase, a protein implicated in the electron transport chain and Krebs cycle. Some mutations have been described in this gene in pheochromocytoma, and this gene could be an appropriate candidate for its study in neuroblastoma given its localization in 1p35-36. The aim of this study was to analyze neuroblastoma tumors in order to assess a possible implication of this gene in neuroblastoma development. We studied 28 neuroblastoma tumor samples from different stages. Mutation research in genomic DNA was carried out after individual amplification of each of the eight SDHB exons by SSCP analysis and sequencing of those samples with migration pattern variants. No variant was found except for three polymorphisms in four neuroblastoma samples. The first polymorphism was a synonymous A → C change in the third position of codon 6 (exon 1). The other two polymorphisms were a TTC insert at the 5′ flanking intron sequence of exon 5 in a stretch of seven TTC repeats. Upon the basis of posterior microsatellite instability and hypermethylation promoter studies, which were not significant, we can conclude that the SDHB gene, a positional candidate gene, is unlikely to be related to either initiation or tumoral progression in neuroblastoma.

Keywords: SDHB gene; Neuroblastoma development; Succinate dehydrogenase

Document Type: Research article

Affiliations: 1: Unidad de Genética y Diagnóstico Prenatal, Hospital Universitario La Fe, Valencia, Spain 2: Servicio de Anatomía Patológica, Hospital Universitario La Fe, Valencia, Spain 3: Unidad de Oncología Pediátrica, Hospital Universitario La Fe, Valencia, Spain

Publication date: 2005-07-01

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• Formerly: Oncology Research Incorporating Anti-Cancer Drug Design
  Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.

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