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Analysis of Inter-(Simple Sequence Repeat) PCR Products From Human Colorectal Cancers

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Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.
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Keywords: Genomic instability; Instability events; Inter-(simple sequence repeat) PCR

Document Type: Research Article

Affiliations: 1: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA 2: Department of Experimental Pathology, Roswell Park Cancer Institute, Buffalo, NY, USA 3: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY, USA 4: MD Anderson Cancer Center, Houston, TX, USA 5: Helen Graham Cancer Center, Wilmington, DE, USA 6: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA, Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA

Publication date: 2005-04-01

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