Authors: Chen, Neng¹; Stoler, Daniel L.²; Dutt, Smitha S.¹; Jahreis, Gerald P.³; Rodriguez-Bigas, Miguel A.⁴; Petrelli, Nicholas J.⁵; Anderson, Garth R.⁶

Source: Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Volume 15, Number 4, 2005 , pp. 233-238(6)

Publisher: Cognizant Communication Corporation

Abstract:

Genomic instability is a fundamental characteristic of solid tumors, and understanding genomic instability should significantly clarify the process of tumorigenesis. We adapted the sampling technique of inter-(simple sequence repeat) PCR [inter-(SSR) PCR] to measure genetic alterations between simple sequence repeats in colorectal tumors. It becomes important to precisely define both normal and altered inter-(SSR) PCR products. BLAT searches of 131 cloned inter-(SSR) PCR sequences reveal that inter-(SSR) PCR products are located on almost all the chromosomes except chromosome Y, indicating that inter-(SSR) PCR samples a representative diverse range of the genome. We confirm that a change in the pattern of the inter-(SSR) PCR products as seen on gel electrophoresis reflects a true alteration within the genome.

Keywords: Inter-(simple sequence repeat) PCR; Genomic instability; Instability events

Document Type: Research article

Affiliations: 1: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA 2: Department of Experimental Pathology, Roswell Park Cancer Institute, Buffalo, NY, USA 3: Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY, USA 4: MD Anderson Cancer Center, Houston, TX, USA 5: Helen Graham Cancer Center, Wilmington, DE, USA 6: Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY, USA, Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY, USA

Publication date: 2005-04-01

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• Formerly: Oncology Research Incorporating Anti-Cancer Drug Design
  Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.

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