Skip to main content

Evaluation of O6-Methylguanine DNA Methyltransferase Activity in Patients With Gastric Cancer

Buy Article:

$79.00 plus tax (Refund Policy)

Abstract:



O6-Methylguanine DNA methyltransferase (O6-MGMT) reverses DNA alkylation damage produced by alkylating agents. O6-MGMT is also a major determinant of cellular resistance to adjuvant chemotherapy with alkylating drugs. O6-MGMT activity was measured in samples from patients with gastric cancer, including tumor, adjacent normal appearing mucosa, and peripheral blood leukocytes (PBL). O6-MGMT activity of PBL from healthy individuals was evaluated as control. There was no significant difference between controls and patients for O6-MGMT activity in PBL. O6-MGMT activity was significantly increased in tumor tissue. Tumor O6-MGMT activity was found to be independent from tumor subgroups and tumor grade. A positive correlation was determined between O6-MGMT activity in tumor and in circulating PBL. The results indicate that O6-MGMT, a defense protein against alkylating agent-mediated carcinogenesis, increased in gastric tumors. This may explain the low response rate to drug combinations, including chloroethylnitrosoureas, exhibited by patients with gastric cancer.

Keywords: Key words: Gastric tumors; Alkylating agents; DNA

Document Type: Research Article

DOI: https://doi.org/10.3727/096504002108748240

Affiliations: 1: *Department of Biochemistry, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, 34300-Turkey 2: †Department of General Surgery, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, 34300-Turkey 3: ‡Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, 34300-Turkey 4: §Department of Pathology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, 34300-Turkey

Publication date: 2002-01-01

More about this publication?
  • Formerly: Oncology Research Incorporating Anti-Cancer Drug Design
    Oncology Research Featuring Preclinical and Clincal Cancer Therapeutics publishes research of the highest quality that contributes to an understanding of cancer in areas of molecular biology, cell biology, biochemistry, biophysics, genetics, biology, endocrinology, and immunology, as well as studies on the mechanism of action of carcinogens and therapeutic agents, reports dealing with cancer prevention and epidemiology, and clinical trials delineating effective new therapeutic regimens.
  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more