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Coamplification of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Genes in Methotrexate-Resistant Human Leukemia Cell Lines

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Abstract:

Methotrexate (MTX)-resistant K562 human myelocytic leukemia sublines with 20- and 200-fold amplified dihydrofolate reductase (DHFR) genes localized to homogeneously staining regions (HSRs) on the long arms of chromosomes 5, 6, and 19 were used to examine whether other genes mapping close to the DHFR genes were coamplified. The gene for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, located on chromosome 5q13.3-14, was coamplified 4–14-fold, corresponding to the levels of resistance exhibited by these cells. Similar observations were made with a MTX-resistant subline of the promyelocytic leukemia cell line, HL-60R, with 200 gene copies of DHFR. These observations indicate a tight linkage of DHFR and HMG-CoA genes on chromosome 5q.

Keywords: Dihydrofolate reductase;; Key words: Methotrexate

Document Type: Research Article

DOI: https://doi.org/10.3727/000000001108747390

Affiliations: 1: *Department of Medicine, Yale University School of Medicine, New Haven, CT 06510 2: †Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, College Park, MD 20742-4450

Publication date: 2001-01-01

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