Stress-Induced Expression of a Novel Variant of Human Fumarate Hydratase (FH)

Authors: LEHTONEN, HELI J.¹; YLISAUKKO-OJA, SANNA K.¹; KIURU, MAIJA¹; KARHU, AULI¹; LEHTONEN, RAINER¹; VANHARANTA, SAKARI¹; JALANKO, ANU²; AALTONEN, LAURI A.¹; LAUNONEN, VIRPI¹

Source: Gene Expression, Volume 14, Number 2, 2007 , pp. 59-69(11)

Publisher: Cognizant Communication Corporation

Buy & download fulltext article:

Price: $79.00 plus tax (Refund Policy)

Abstract:

Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon 1b, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.

Keywords: Tricarboxylic acid cycle (TCAC); Fumarate hydratase (FH); Alternative exon; Hereditary leiomyomatosis and renal cell cancer (H; Hypoxia; Heat shock; Stress

Document Type: Research article

DOI: http://dx.doi.org/10.3727/105221607783417592

Affiliations: 1: Department of Medical Genetics, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland 2: National Public Health Institute, Department of Molecular Medicine, Biomedicum Helsinki, Helsinki, Finland

Publication date: 2007-02-01

More about this publication?

The Molecular and Cellular Biology area of Gene Expression covers all aspects of the gene including it structure, functions, and regulation in prokaryotes, eukaryotes, and viruses; molecular and cell biological aspects of cell growth and development, chromatin structure and function. These include topics such as DNA replication, DNA repair, gene transcription, transcriptional control, RNA processing, posttranscriptional control, oncogenes, molecular mechanisms of action of hormones, molecular mechanism of cellular differentiation, growth and development, protein synthesis, and posttranslational control.
The Molecular and Cellular Neuroscience area of Gene Expression covers all aspects of gene expression as described but is devoted exclusively to the nervous system in health and disease. Topics include studies of neurogenesis, development, aging, and neurodegeneration. Complex neural systems, motor control, special senses, and higher cortical function, when viewed from the perspective of gene expression, are appropriate for the journal. Research related to molecular mechanisms of drug tolerance, dependence, and withdrawal are solicited. Manuscripts on state-of-the-art methods and protocols for molecular profiling of neuronal structure and function are welcome.

Stress-Induced Expression of a Novel Variant of Human Fumarate Hydratase (FH)

Buy & download fulltext article:

Tools

Key

Text size:

Useful pages