Shopping cart
RADIO FREQUENCY ENERGY LOOP PRIMES CARDIAC, NEURONAL, AND SKELETAL MUSCLE DIFFERENTIATION IN MOUSE EMBRYONIC STEM CELLS: A NEW TOOL FOR IMPROVING TISSUE REGENERATION
Authors: Margherita Maioli, Salvatore Rinaldi, Sara Santaniello, Alessandro Castagna, Gianfranco Pigliaru, Sara Gualini, Vania Fontani, Carlo Ventura
Source: Cell Transplantation
Publisher: Cognizant Communication Corporation
Buy & download fulltext article:
The full text is free.
View now:
Abstract:
Radiofrequency (RF) waves from Wi-Fi (Wireless Fidelity) technologies have become ubiquitous, with Internet access spreading into homes, and public areas. The human body harbors multipotent stem cells with various grading of potentiality. Whether stem cells may be affected by Wi-Fi RF energy remains unknown.We exposed mouse embryonic stem (ES) cells to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering Wi-Fi RF of 2.4 GHz with its conveyer electrodes immersed into the culture medium. Cell responses were investigated by real-time PCR, Western blot and confocal microscopy. Single RF burst duration, Radiated power, Electric and Magnetic fields, Specific Absorption Rate, and current density in culture medium were monitored. REAC stimulation primed transcription of genes involved in cardiac (GATA4, Nkx-2.5 and prodynorphin), skeletal muscle (myoD) and neuronal (neurogenin1) commitment, while downregulating the self renewal/pluripotency-associated genes Sox2, Oct4 and Nanog.REAC exposure enhanced the expression of cardiac, skeletal and neuronal lineagerestricted marker proteins. The number of spontaneously beating ES-derived myocardial cells was also increased.In conclusion, REAC stimulation provided a "physical milieu" optimizing stem cell expression of pluripotentiality and the attainment of three major target lineages for regenerative medicine, without using chemical agonists or vector-mediated gene delivery.Document Type:
DOI: http://dx.doi.org/10.3727/096368911X600966
Appeared or available online: September 22, 2011
Key
Print this page