EFFECTS OF MYELOABLATION, PERIPHERAL CHIMERISM AND WHOLE BODY IRRADIATION ON THE ENTRY OF BONE MARROW-DERIVED CELLS INTO THE BRAIN

Authors: Antoine Lampron, Martine Lessard, Serge Rivest

Source: Cell Transplantation

Publisher: Cognizant Communication Corporation

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Abstract:

how bone marrow-derived cells (BMDCs) enter the central nervous system (CNS) is critical for the development of therapies for brainrelated disorders using hematopoietic stem cells. We investigated the brain damages and blood-brain barrier (BBB) modification following either whole-body irradiation or a myeloablative chemotherapy regimen in mice, and the capacity for these treatments to induce the entry of BMDCs into the CNS. Neither treatment had a lasting effect on brain integrity and both were equally efficient at achieving myeloablation. Injection of bone marrow cells from green fluorescent protein (GFP) transgenic mice was able to completely repopulate the hematopoietic niche in the circulation and in hematopoietic organs (thymus and spleen). However, GFP+ cells only entered the brain following whole-body irradiation. We conclude that myeloablation, damages to the brain integrity or the BBB and peripheral chimerism are not responsible for the entry of BMDCs into the CNS following irradiation.

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DOI: http://dx.doi.org/10.3727/096368911X593154

Appeared or available online: September 22, 2011