Characteristics of mouse adipose tissue-derived stem cells and therapeutic comparison between syngeneic and allogeneic adipose tissue-derived stem cell transplantation in experimental autoimmune thyroiditis
Author: Eun Wha Choi, Il Seob Shin, So Young Park, Eun Ji Yoon, Sung Keun Kang, Jeong Chan Ra, Sung Hwa Hong
Source: Cell Transplantation
Publisher: Cognizant Communication Corporation
Abstract:Previously, we found that the intravenous administration of human adipose tissue-derived mesenchymal stem cells was a promising therapeutic option for autoimmune thyroiditis even when the cells were transplanted into a xenogeneic model without an immunosuppressant. Therefore, we explored the comparison between the therapeutic effects of syngeneic and allogeneic adipose tissue-derived stem cells on an experimental autoimmune thyroiditis mouse model. Experimental autoimmune thyroiditis was induced in C57BL/6 mice by immunization with porcine thyroglobulin. Adipose tissue-derived stem cells derived from C57BL/6 mice (syngeneic) or BALB/c mice (allogeneic), or saline as a vehicle control were administered intravenously 4 times weekly. Blood and tissue samples were collected 1 week after the last transplantation. Adipose tissue-derived stem cells from mice were able to differentiate into multiple lineages in vitro; however, mouse adipose tissue-derived stem cells did not have immunophenotypes identical to those from humans. Administrations of adipose tissue-derived stem cells, whether syngeneic or allogeneic, reduced thyroglobulin autoantibodies and the inflammatory immune response protected against lymphocytes infiltration into the thyroid and restored the Th1/Th2 balance, without any adverse effects. However, different humoral immune responses were observed for infused cells from different stem cell sources. The strongest humoral immune response was induced by xenogeneic transplantation, followed by allogeneic and syngeneic administration, in that order. The stem cells were mostly found in the spleen, not the thyroid. This migration might be because the stem cells primarily function in systemic immune modulation, due to being given prior to disease induction. In this study, we confirmed the effects of adipose tissue-derived stem cells in treating autoimmune thyroiditis without differences between syngeneic and allogeneic transplantation.
Appeared or available online: March 5, 2013